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KIF20B Polyclonal antibody

KIF20B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27269-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 90, CT90, KIF20B, kinesin family member 20B, Kinesin like protein KIF20B, KRMP1, M phase phosphoprotein 1, MPHOSPH1, MPP 1, MPP1

验证数据展示

  • IHC staining of human colon cancer using 27269-1-AP

    IHC staining of human colon cancer using 27269-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27269-1-AP

    IHC staining of human colon cancer using 27269-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27269-1-AP

    WB analysis using 27269-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27269-1-AP (KIF20B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHT-29 cells, mouse brain tissue, HepG2 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27269-1-AP is KIF20B Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, Mouse
Immunogen KIF20B fusion protein Ag26191 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 20B
Synonyms Cancer/testis antigen 90, CT90, KIF20B, kinesin family member 20B, Kinesin like protein KIF20B, KRMP1, M phase phosphoprotein 1, MPHOSPH1, MPP 1, MPP1
Calculated Molecular Weight 211 kDa
Observed Molecular Weight214 kDa
GenBank Accession NumberNM_001284259
Gene Symbol KIF20B
Gene ID (NCBI) 9585
RRIDAB_2918121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF20B antibody 27269-1-APDownload protocol
IHC protocol for KIF20B antibody 27269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols