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KIF21A Polyclonal antibody

KIF21A Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27276-1-AP

Print datasheet

Synonyms

KIF2, KIAA1708, FEOM3A, FEOM1, CFEOM1

验证数据展示

  • WB analysis using 27276-1-AP

    WB analysis using 27276-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27276-1-AP (KIF21A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 27276-1-AP

    WB analysis of mouse brain using 27276-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27276-1-AP (KIF21A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 27276-1-AP

    IP experiment of mouse brain using 27276-1-AP

    IP result of anti-KIF21A (IP:27276-1-AP, 4ug; Detection:27276-1-AP 1:500) with mouse brain tissue lysate 5600ug.

  • IHC staining of human ovary cancer using 27276-1-AP

    IHC staining of human ovary cancer using 27276-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27276-1-AP (KIF21A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, mouse brain tissue, MCF-7
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27276-1-AP targets KIF21A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityrat
Immunogen KIF21A fusion protein Ag26192 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 21A
Synonyms KIF2, KIAA1708, FEOM3A, FEOM1, CFEOM1
Calculated Molecular Weight 187 kDa
Observed Molecular Weight 190-200 kDa
GenBank Accession NumberNM_001173463
Gene Symbol KIF21A
Gene ID (NCBI) 55605
RRIDAB_2880825
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF21A is well known for its association with congenital fibrosis of the extraocular muscles type 1 (CFEOM1). CFEOM1-related mutations in KIF21A relieve autoinhibition of the motor and third coiled-coil stalk, which results in enhanced accumulation of KIF21A in axonal growth cones and aberrant axon morphology in the oculomotor nerve.(PMID: 38767486)

实验方案

Product Specific Protocols
WB protocol for KIF21A antibody 27276-1-APDownload protocol
IHC protocol for KIF21A antibody 27276-1-APDownload protocol
IP protocol for KIF21A antibody 27276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

Neural Regen Res

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

Authors - Shi-Yan Sun
View Article