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验证数据展示

  • WB analysis using 13403-1-AP

    WB analysis using 13403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 13403-1-AP

    IP experiment of HeLa using 13403-1-AP

    IP result of anti-KIF22 (IP:13403-1-AP, 4ug; Detection:13403-1-AP 1:300) with HeLa cells lysate 4000ug.

  • IHC staining of human brown disease using 13403-1-AP

    IHC staining of human brown disease using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 13403-1-AP (KIF22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of human cervical cancer using 13403-1-AP

    IHC staining of human cervical cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of human cervical cancer using 13403-1-AP

    IHC staining of human cervical cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of human stomach cancer using 13403-1-AP

    IHC staining of human stomach cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of mouse testis using 13403-1-AP

    IHC staining of mouse testis using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of mouse testis using 13403-1-AP

    IHC staining of mouse testis using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of human stomach cancer using 13403-1-AP

    IHC staining of human stomach cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • Featured Product
  • KD/KO验证

KIF22 Polyclonal antibody

KIF22 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13403-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

别名

Kinesin-like protein KIF22, Kinesin-like protein 4, Kinesin-like DNA-binding protein, Kinesin like protein KIF22, Kinesin like protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, human heart tissue, MKN-45 cells, T-47D cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human breast cancer tissue, human brown disease, human cervical cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KIF22 Ready-To-Use IHC Kit. KIF22 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13403-1-AP targets KIF22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 KIF22 fusion protein Ag4254 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 22
别名 Kinesin-like protein KIF22, Kinesin-like protein 4, Kinesin-like DNA-binding protein, Kinesin like protein KIF22, Kinesin like protein 4
计算分子量 665 aa, 73 kDa
观测分子量 68-73 kDa
GenBank蛋白编号BC028155
基因名称 KIF22
Gene ID (NCBI) 3835
RRIDAB_2249576
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14807
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).

实验方案

Product Specific Protocols
WB protocol for KIF22 antibody 13403-1-APDownload protocol
IHC protocol for KIF22 antibody 13403-1-APDownload protocol
IP protocol for KIF22 antibody 13403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Sci Rep

PMID: 29229923

Authors - Sara Bizzotto
View Article
humanWB

Proteomics

Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.

Authors - Yan Guang-Rong GR
View Article
humanWB,IHC

Chin Med J (Engl)

Effect of KIF22 on promoting proliferation and migration of gastric cancer cells via MAPK-ERK pathways.

Authors - Ze-Yuan Yu
  • KD Validated
View Article