• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 13403-1-AP

    WB analysis using 13403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 13403-1-AP

    IP experiment of HeLa using 13403-1-AP

    IP result of anti-KIF22 (IP:13403-1-AP, 4ug; Detection:13403-1-AP 1:300) with HeLa cells lysate 4000ug.

  • IHC staining of human brown disease using 13403-1-AP

    IHC staining of human brown disease using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 13403-1-AP (KIF22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of human cervical cancer using 13403-1-AP

    IHC staining of human cervical cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of human cervical cancer using 13403-1-AP

    IHC staining of human cervical cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of human stomach cancer using 13403-1-AP

    IHC staining of human stomach cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of mouse testis using 13403-1-AP

    IHC staining of mouse testis using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of mouse testis using 13403-1-AP

    IHC staining of mouse testis using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • IHC staining of human stomach cancer using 13403-1-AP

    IHC staining of human stomach cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13403-1-AP (KIF22 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF22 antibody included
  • Featured Product
  • KD/KO Validated

KIF22 Polyclonal antibody

KIF22 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13403-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (1)

Synonyms

Kinesin-like protein KIF22, Kinesin-like protein 4, Kinesin-like DNA-binding protein, Kinesin like protein KIF22, Kinesin like protein 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, human heart tissue, MKN-45 cells, T-47D cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human breast cancer tissue, human brown disease, human cervical cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KIF22 Ready-To-Use IHC Kit. KIF22 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13403-1-AP targets KIF22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen KIF22 fusion protein Ag4254 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 22
Synonyms Kinesin-like protein KIF22, Kinesin-like protein 4, Kinesin-like DNA-binding protein, Kinesin like protein KIF22, Kinesin like protein 4
Calculated Molecular Weight 665 aa, 73 kDa
Observed Molecular Weight 68-73 kDa
GenBank Accession NumberBC028155
Gene Symbol KIF22
Gene ID (NCBI) 3835
RRIDAB_2249576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14807
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).

实验方案

Product Specific Protocols
WB protocol for KIF22 antibody 13403-1-APDownload protocol
IHC protocol for KIF22 antibody 13403-1-APDownload protocol
IP protocol for KIF22 antibody 13403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Sci Rep

PMID: 29229923

Authors - Sara Bizzotto
View Article
humanWB

Proteomics

Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.

Authors - Yan Guang-Rong GR
View Article
humanWB,IHC

Chin Med J (Engl)

Effect of KIF22 on promoting proliferation and migration of gastric cancer cells via MAPK-ERK pathways.

Authors - Ze-Yuan Yu
  • KD Validated
View Article