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KIF22 Polyclonal antibody
KIF22 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human heart tissue, MKN-45 cells, T-47D cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, human breast cancer tissue, human brown disease, human cervical cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy KIF22 Ready-To-Use IHC Kit. KIF22 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13403-1-AP targets KIF22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | KIF22 fusion protein Ag4254 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | kinesin family member 22 |
| Synonyms | Kinesin-like protein KIF22, Kinesin-like protein 4, Kinesin-like DNA-binding protein, Kinesin like protein KIF22, Kinesin like protein 4 |
| Calculated Molecular Weight | 665 aa, 73 kDa |
| Observed Molecular Weight | 68-73 kDa |
| GenBank Accession Number | BC028155 |
| Gene Symbol | KIF22 |
| Gene ID (NCBI) | 3835 |
| RRID | AB_2249576 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14807 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF22 antibody 13403-1-AP | Download protocol |
| IHC protocol for KIF22 antibody 13403-1-AP | Download protocol |
| IP protocol for KIF22 antibody 13403-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proteomics Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study. | ||
Chin Med J (Engl) Effect of KIF22 on promoting proliferation and migration of gastric cancer cells via MAPK-ERK pathways.
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