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验证数据展示

  • IHC staining of human stomach cancer using 28587-1-AP (same clone as 28587-1-PBS)

    IHC staining of human stomach cancer using 28587-1-AP (same clone as 28587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

  • WB analysis using 28587-1-AP (same clone as 28587-1-PBS)

    WB analysis using 28587-1-AP (same clone as 28587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28587-1-AP (KIF23 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 28587-1-AP (same clone as 28587-1-PBS)

    IF Staining of HepG2 using 28587-1-AP (same clone as 28587-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIF23 antibody (28587-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KIF23 Polyclonal antibody, PBS Only

KIF23 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28587-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MKLP1, KNSL5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

28587-1-PBS targets KIF23 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 KIF23 fusion protein Ag29346 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 23
别名 MKLP1, KNSL5
计算分子量 110 kDa
观测分子量110 kDa
GenBank蛋白编号NM_138555
基因名称 KIF23
Gene ID (NCBI) 9493
RRIDAB_2881176
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02241
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIF23, also known as KNSL5 and MKLP1, is a member of the kinesin-like protein family. It plays a crucial role in cytokinesis, the final stage of cell division. It is involved in the formation of the central spindle, which is essential for the proper separation of the cytoplasm and the formation of the cleavage furrow. KIF23 is also involved in the organization and transport of intracellular vesicles and organelles. Mutations in KIF23 have been associated with congenital dyserythropoietic anemia type III(PMID: 23570799).