• Featured Product
  • KD/KO Validated

KIF2A Polyclonal antibody

KIF2A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 13105-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Kinesin-2, Kinesin like protein KIF2A, kinesin heavy chain member 2A, Kinesin 2, KIF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, MDCK cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13105-1-AP targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Immunogen KIF2A fusion protein Ag3748 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin heavy chain member 2A
Synonyms Kinesin-2, Kinesin like protein KIF2A, kinesin heavy chain member 2A, Kinesin 2, KIF2
Calculated Molecular Weight 679 aa, 77 kDa
Observed Molecular Weight 80-100 kDa
GenBank Accession NumberBC031828
Gene Symbol KIF2A
Gene ID (NCBI) 3796
RRIDAB_2265336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00139
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF2A antibody 13105-1-APDownload protocol
IHC protocol for KIF2A antibody 13105-1-APDownload protocol
IF protocol for KIF2A antibody 13105-1-APDownload protocol
IP protocol for KIF2A antibody 13105-1-APDownload protocol
FC protocol for KIF2A antibody 13105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network

Authors - Cuirong Guan
  • KD Validated
humanWB, IF

J Cell Biol

WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.

Authors - Junjie Huang
mouseWB,IF

EMBO Rep

Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis

Authors - Jia-Qian Ju
humanWB

Front Oncol

Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.

Authors - Xigang Xia
humanWB

Biomed Pharmacother

KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.

Authors - Xin Zhang
  • KD Validated
humanIF

Mol Biol Cell

Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.

Authors - Hongdan Yang
Loading...