验证数据展示
经过测试的应用
Positive WB detected in | DU 145 cells, PC-3 cells, mouse testis, rat testis |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 7 publications below |
产品信息
28372-1-AP targets KIF2C in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | Human, mouse, rat |
文献引用反应性 | human |
免疫原 | KIF2C fusion protein Ag29009 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | kinesin family member 2C |
别名 | KIF2C, kinesin family member 2C, Kinesin like protein 6, Kinesin like protein KIF2C, KNSL6, MCAK |
计算分子量 | 725 aa, 81 kDa |
观测分子量 | 80 kDa |
GenBank蛋白编号 | BC014924 |
基因名称 | KIF2C |
Gene ID (NCBI) | 11004 |
RRID | AB_2881127 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99661 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for KIF2C antibody 28372-1-AP | Download protocol |
IHC protocol for KIF2C antibody 28372-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Transl Res Identification of biomarkers for the transition from low-grade glioma to secondary glioblastoma by an integrated bioinformatic analysis. | ||
Front Genet KIF2C is a Biomarker Correlated With Prognosis and Immunosuppressive Microenvironment in Human Tumors. | ||
Am J Cancer Res Multi-omics analysis of kinesin family member 2C in human tumors: novel prognostic biomarker and tumor microenvironment regulator | ||
J Cancer Res Clin Oncol Development and validation of a vesicle-mediated transport-associated gene signature for predicting prognosis and immune therapy response in hepatocellular carcinoma | ||
Sci Rep Bioinformatical analysis of the key differentially expressed genes for screening potential biomarkers in Wilms tumor | ||
Pharmaceuticals (Basel) Identification of Key Immune and Cell Cycle Modules and Prognostic Genes for Glioma Patients through Transcriptome Analysis |