验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human cerebellum tissue, mouse cerebellum tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13930-1-AP targets KIF3A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | KIF3A fusion protein Ag4954 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin family member 3A |
| 别名 | KIF3, KIF3A, kinesin family member 3A, Kinesin like protein KIF3A |
| 计算分子量 | 80 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC045542 |
| 基因名称 | KIF3A |
| Gene ID (NCBI) | 11127 |
| RRID | AB_2132063 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y496 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate. (23386061)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF3A antibody 13930-1-AP | Download protocol |
| IHC protocol for KIF3A antibody 13930-1-AP | Download protocol |
| IP protocol for KIF3A antibody 13930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis. | ||
Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. | ||
Proc Natl Acad Sci U S A EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.
| ||
EMBO J Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.
| ||
Cell Mol Life Sci Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.
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