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验证数据展示

  • IHC staining of human lung cancer using 14344-1-AP

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 14344-1-AP

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 14344-1-AP

    IF Staining of HepG2 using 14344-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIF4A antibody 14344-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

KIF4A Polyclonal antibody

KIF4A Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 14344-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

KIF4 G1, KIF4, HSA271784, Chromosome-associated kinesin KIF4A, Chromokinesin-A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14344-1-AP targets KIF4A in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KIF4A fusion protein Ag5682 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 4A
别名 KIF4 G1, KIF4, HSA271784, Chromosome-associated kinesin KIF4A, Chromokinesin-A
计算分子量 140 kDa
GenBank蛋白编号BC050548
基因名称 KIF4A
Gene ID (NCBI) 24137
RRIDAB_2132200
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95239
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for KIF4A antibody 14344-1-APDownload protocol
IF protocol for KIF4A antibody 14344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols