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KIF4A Polyclonal antibody

KIF4A Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14344-1-AP

Print datasheet

Synonyms

KIF4 G1, KIF4, HSA271784, Chromosome-associated kinesin KIF4A, Chromokinesin-A

验证数据展示

  • IHC staining of human lung cancer using 14344-1-AP

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 14344-1-AP

    IHC staining of human lung cancer using 14344-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 14344-1-AP (KIF4A antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 14344-1-AP

    IF Staining of HepG2 using 14344-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIF4A antibody 14344-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14344-1-AP targets KIF4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen KIF4A fusion protein Ag5682 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 4A
Synonyms KIF4 G1, KIF4, HSA271784, Chromosome-associated kinesin KIF4A, Chromokinesin-A
Calculated Molecular Weight 140 kDa
GenBank Accession NumberBC050548
Gene Symbol KIF4A
Gene ID (NCBI) 24137
RRIDAB_2132200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95239
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for KIF4A antibody 14344-1-APDownload protocol
IF protocol for KIF4A antibody 14344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Clin Cancer Res

Targeting the KIF4A/AR axis to reverse endocrine therapy resistance in castration-resistant prostate cancer.

Authors - Ke Chen
View Article
humanWB,IHC

Anal Cell Pathol (Amst)

Aspirin Exerts Its Antitumor Effect in Esophageal Squamous Cell Carcinoma by Downregulating the Expression of ATAD2 and KIF4A

Authors - Man Zhang
View Article