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KIF5C Polyclonal antibody

KIF5C Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25897-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

NKHC, KINN, Kinesin-1, Kinesin heavy chain neuron-specific 2, KIAA0531

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25897-1-AP targets KIF5C in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 KIF5C fusion protein Ag23163 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 5C
别名 NKHC, KINN, Kinesin-1, Kinesin heavy chain neuron-specific 2, KIAA0531
计算分子量 725 aa, 83 kDa
观测分子量 70 kDa, 110 kDa
GenBank蛋白编号BC110287
基因名称 KIF5C
Gene ID (NCBI) 3800
RRIDAB_2880288
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60282
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF5C (Kinesin heavy chain isoform 5C variant) which belongs to the kinesin-like protein family is neuron specific. The kinesin-1 subfamilies (kif5a, kif5b, kif5c) are responsible for the movement of mitochondria in neurons. Kinesins have N-terminal motor domains and C-terminal cargo-binding tail domains separated by hinge regions. The hinge and C-terminal tail regions of Kif5a, Kif5b, and Kif5c bound a large detergent-resistant RNase-sensitive granule. The granule localized to dendrites and underwent bidirectional movement. Distally directed movement of the granule was enhanced by Kif5 overexpression and reduced by Kif5 functional blockage. kinesins may transport RNA in dendrites via this large granule. Two major isoforms of kif5c, with molecular predicted masses of 80 and 110 kDa, are generated by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for KIF5C antibody 25897-1-APDownload protocol
IHC protocol for KIF5C antibody 25897-1-APDownload protocol
IF protocol for KIF5C antibody 25897-1-APDownload protocol
IP protocol for KIF5C antibody 25897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A

Authors - Atul Kumar
mouseWB

Exp Neurol

Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI

Authors - Gang Liu
humanWB

Hum Mol Genet

Mitochondrial transport mediates survival of retinal ganglion cells in affected LHON patients.

Authors - Tien-Chun Yang
mouseWB

Alzheimers Res Ther

Normal levels of KIF5 but reduced KLC1 levels in both Alzheimer disease and Alzheimer disease in Down syndrome: evidence suggesting defects in anterograde transport.

Authors - Xu-Qiao Chen
humanWB

bioRxiv

MFN2-dependent recruitment of ATAT1 coordinates mitochondria motility with alpha-tubulin acetylation and is disrupted in CMT2A

Authors - Atul Kumar
humanWB,IHC

Int Endod J

Overexpression of MicroRNA-155 Aggravates Pulpitis by Targeting Kinesin Superfamily Proteins-5C Based on Illumina High-Throughput Sequencing

Authors - Ye Jiang
  • KD Validated
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