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KIF6 Polyclonal antibody

KIF6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17290-1-AP

Print datasheet

Synonyms

C6orf102, KIF6, kinesin family member 6, Kinesin like protein KIF6

验证数据展示

  • WB analysis using 17290-1-AP

    WB analysis using 17290-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17290-1-AP (KIF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17290-1-AP

    IHC staining of human liver using 17290-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17290-1-AP (KIF6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 17290-1-AP

    IHC staining of human liver using 17290-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17290-1-AP (KIF6 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of mouse testis using 17290-1-AP

    IP experiment of mouse testis using 17290-1-AP

    IP result of anti-KIF6 (IP:17290-1-AP, 4ug; Detection:17290-1-AP 1:500) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17290-1-AP targets KIF6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, monkey
Immunogen KIF6 fusion protein Ag11164 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 6
Synonyms C6orf102, KIF6, kinesin family member 6, Kinesin like protein KIF6
Calculated Molecular Weight265aa,30 kDa; 814aa,93 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC022074
Gene Symbol KIF6
Gene ID (NCBI) 221458
RRIDAB_2296657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZMV9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF6 antibody 17290-1-APDownload protocol
IHC protocol for KIF6 antibody 17290-1-APDownload protocol
IP protocol for KIF6 antibody 17290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,monkeyWB,IF

bioRxiv

Kif6 regulates cilia motility and polarity in brain ependymal cells

Authors - Maki Takagishi
View Article
mouseIF,WB

Dis Model Mech

Motor protein Kif6 regulates cilia motility and polarity in brain ependymal cells

Authors - Maki Takagishi
View Article