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验证数据展示

  • WB analysis using 17290-1-AP

    WB analysis using 17290-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17290-1-AP (KIF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17290-1-AP

    IHC staining of human liver using 17290-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17290-1-AP (KIF6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 17290-1-AP

    IHC staining of human liver using 17290-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17290-1-AP (KIF6 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of mouse testis using 17290-1-AP

    IP experiment of mouse testis using 17290-1-AP

    IP result of anti-KIF6 (IP:17290-1-AP, 4ug; Detection:17290-1-AP 1:500) with mouse testis tissue lysate 4000ug.

KIF6 Polyclonal antibody

KIF6 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17290-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IP, IHC, ELISA and More (1)

别名

C6orf102, KIF6, kinesin family member 6, Kinesin like protein KIF6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17290-1-AP targets KIF6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse, monkey
免疫原 KIF6 fusion protein Ag11164 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 6
别名 C6orf102, KIF6, kinesin family member 6, Kinesin like protein KIF6
计算分子量265aa,30 kDa; 814aa,93 kDa
观测分子量 93 kDa
GenBank蛋白编号BC022074
基因名称 KIF6
Gene ID (NCBI) 221458
RRIDAB_2296657
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZMV9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF6 antibody 17290-1-APDownload protocol
IHC protocol for KIF6 antibody 17290-1-APDownload protocol
IP protocol for KIF6 antibody 17290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,monkeyWB,IF

bioRxiv

Kif6 regulates cilia motility and polarity in brain ependymal cells

Authors - Maki Takagishi
View Article
mouseIF,WB

Dis Model Mech

Motor protein Kif6 regulates cilia motility and polarity in brain ependymal cells

Authors - Maki Takagishi
View Article