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  • Featured Product
  • KD/KO Validated

KIFAP3 Polyclonal antibody

KIFAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12700-1-AP

Print datasheet

Synonyms

Kinesin-associated protein 3, kinesin associated protein 3, KIF3AP, KAP-3, KAP3

验证数据展示

  • IF Staining of HepG2 using 12700-1-AP

    IF Staining of HepG2 using 12700-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12700-1-AP (KIFAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human brain using 12700-1-AP

    WB analysis of human brain using 12700-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12700-1-AP (KIFAP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 12700-1-AP

    WB analysis of MCF-7 using 12700-1-AP

    WB result of KIFAP3 antibody (12700-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIFAP3 transfected MCF-7 cells.

  • IP experiment of mouse testis using 12700-1-AP

    IP experiment of mouse testis using 12700-1-AP

    IP result of anti-KIFAP3 (IP:12700-1-AP, 4ug; Detection:12700-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • IHC staining of human ovary cancer using 12700-1-AP

    IHC staining of human ovary cancer using 12700-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12700-1-AP (KIFAP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue, mouse testis tissue, mouse brain tissue, MCF-7 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12700-1-AP targets KIFAP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen KIFAP3 fusion protein Ag3394 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin-associated protein 3
Synonyms Kinesin-associated protein 3, kinesin associated protein 3, KIF3AP, KAP-3, KAP3
Calculated Molecular Weight 792 aa, 91 kDa
Observed Molecular Weight 91-100 kDa
GenBank Accession NumberBC028679
Gene Symbol KIFAP3
Gene ID (NCBI) 22920
RRIDAB_2132528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92845
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIFAP3, also known as KIF3AP or KAP3, is a novel KIF3A/3B-associated protein. It binds to the tail domain of KIF3A/3B and may play a role in regulating the binding of KIF3A/3B to cargoes. Recently it has been reported that mutation within the KIFAP3 gene is associated with decreased KIFAP3 expression and increased survival in sporadic ALS, which makes it a potential target for ALS therapy. KIFAP3 has also been identified as the target of mir-130a.

实验方案

Product Specific Protocols
WB protocol for KIFAP3 antibody 12700-1-APDownload protocol
IHC protocol for KIFAP3 antibody 12700-1-APDownload protocol
IF protocol for KIFAP3 antibody 12700-1-APDownload protocol
IP protocol for KIFAP3 antibody 12700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

A functional assay for microRNA target identification and validation.

Authors - G?ken Joop J
View Article
humanWB

Commun Biol

The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells

Authors - Christopher Noyes
View Article