验证数据展示
经过测试的应用
| Positive WB detected in | rat testis tissue, HeLa cells, mouse testis tissue, NIH/3T3 cells |
| Positive IHC detected in | human bowen disease, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20790-1-AP targets KIFC1 in WB, IHC, IF/ICC, ELISA, Peptide array applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, Peptide array |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | KIFC1 fusion protein Ag14492 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin family member C1 |
| 别名 | KNSL2, Kinesin-related protein HSET, Kinesin-like protein KIFC1, Kinesin-like protein 2, HSET |
| 计算分子量 | 673 aa, 74 kDa |
| 观测分子量 | 55-70 kDa |
| GenBank蛋白编号 | BC000712 |
| 基因名称 | KIFC1 |
| Gene ID (NCBI) | 3833 |
| RRID | AB_10755286 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BW19 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport. This antibody was raised against the C-terminal region of human KIFC1 protein, and recognizes 55-70 kDa of endogenous KIFC1 protein in multiple tissue lysates. (12826589, 15236353, 23543461)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIFC1 antibody 20790-1-AP | Download protocol |
| IHC protocol for KIFC1 antibody 20790-1-AP | Download protocol |
| IF protocol for KIFC1 antibody 20790-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Mechanism of spindle pole organization and instability in human oocytes.
| ||
Dev Cell Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds. | ||
Cell Mol Life Sci Proteomic interaction profiling reveals KIFC1 as a factor involved in early targeting of F508del-CFTR to degradation.
| ||
FASEB J Export of membrane proteins from the Golgi complex to the primary cilium requires the kinesin motor, KIFC1. | ||
Front Mol Biosci Prognostic Value and Immunological Role of KIFC1 in Hepatocellular Carcinoma. |
