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- KD/KO Validated
KIFC3 Polyclonal antibody
KIFC3 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, mouse kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10125-2-AP targets KIFC3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, monkey |
| Immunogen | KIFC3 fusion protein Ag0172 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | kinesin family member C3 |
| Synonyms | Kinesin-like protein KIFC3 |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 80-85 kDa, 95-100 kDa |
| GenBank Accession Number | BC001211 |
| Gene Symbol | KIFC3 |
| Gene ID (NCBI) | 3801 |
| RRID | AB_2296476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIFC3 antibody 10125-2-AP | Download protocol |
| IHC protocol for KIFC3 antibody 10125-2-AP | Download protocol |
| IP protocol for KIFC3 antibody 10125-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner | ||
EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
| ||
Mol Biol Cell Kif5B and Kifc1 interact and are required for motility and fission of early endocytic vesicles in mouse liver. | ||
World J Gastrointest Oncol KIFC3 promotes proliferation, migration and invasion of esophageal squamous cell carcinoma cells by activating EMT and β-catenin signaling
| ||
Biochim Biophys Acta The iron-regulated metastasis suppressor, Ndrg-1: identification of novel molecular targets. |
