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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 32059-1-AP

    WB analysis using 32059-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32059-1-AP (KIR2DL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

KIR2DL4/CD158d Polyclonal antibody

KIR2DL4/CD158d Polyclonal Antibody for WB, ELISA
Cat No. 32059-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KIR2DL4, KIR103, KIR 103AS, Killer cell immunoglobulin-like receptor 2DL4, G9P

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, NK-92 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32059-1-AP targets KIR2DL4/CD158d in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
Synonyms KIR2DL4, KIR103, KIR 103AS, Killer cell immunoglobulin-like receptor 2DL4, G9P
Calculated Molecular Weight 377 aa, 42 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC041611
Gene Symbol KIR2DL4
Gene ID (NCBI) 3805
RRIDAB_3670180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ99706
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells that regulate their responsiveness to signals received from their environment (PMID: 22934097). Killer cell immunoglobulin-like receptor 2DL4 (KIR2DL4, also known as CD158D and KIR103AS) is an unusual member of the KIRs family, which activates the cytotoxicity of NK cells. KIR2DL4 has two immunoglobulin domains and a single immunoreceptor tyrosine-based inhibition motif (ITIM) in the cytoplasmic tail with weak inhibitory potential (PMID: 12055234).

实验方案

Product Specific Protocols
WB protocol for KIR2DL4/CD158d antibody 32059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols