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验证数据展示

  • WB analysis using 22232-1-AP

    WB analysis using 22232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22232-1-AP (KIRREL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 22232-1-AP

    IF Staining of mouse kidney using 22232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KIRREL antibody (22232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIRREL Polyclonal antibody

KIRREL Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 22232-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

Nephrin-like protein 1, NEPH1, KIRREL1, Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC2C12 cells, mouse brain tissue, mouse kidney tissue, mouse lung tissue, rat brain tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22232-1-AP targets KIRREL in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KIRREL fusion protein Ag17578 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kin of IRRE like (Drosophila)
别名 Nephrin-like protein 1, NEPH1, KIRREL1, Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1
计算分子量 757 aa, 84 kDa
观测分子量72 kDa
GenBank蛋白编号BC109192
基因名称 KIRREL
Gene ID (NCBI) 55243
RRIDAB_3085689
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96J84
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kin of IRRE protein (KIRREL), also known as NEPH1, is a member of a podocin-binding protein family. KIRREL is a component of the podocyte slit diaphragm, which serves as the structural framework of the glomerular filtration barrier. KIRREL has three isoforms with molecular weights of 85 kDa, 83 kDa, and 72 kDa.

实验方案

Product Specific Protocols
WB protocol for KIRREL antibody 22232-1-APDownload protocol
IF protocol for KIRREL antibody 22232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols