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  • Featured Product
  • KD/KO Validated

KLC1 Polyclonal antibody

KLC1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat No : 19028-1-AP

Print datasheet

Synonyms

kinesin light chain 1, KLC, KLC 1, KLC1, KNS2, KNS2A

验证数据展示

  • WB analysis of mouse brain using 19028-1-AP

    WB analysis of mouse brain using 19028-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19028-1-AP

    IHC staining of human brain using 19028-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 19028-1-AP

    IHC staining of human brain using 19028-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 19028-1-AP

    IF Staining of HeLa using 19028-1-AP

    Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inmouse brain tissue, COLO 320 cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 19028-1-AP is KLC1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen KLC1 fusion protein Ag13570 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin light chain 1
Synonyms kinesin light chain 1, KLC, KLC 1, KLC1, KNS2, KNS2A
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 61-70 kDa
GenBank Accession NumberBC008881
Gene Symbol KLC1
Gene ID (NCBI) 3831
RRIDAB_10641041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07866
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLC1 antibody 19028-1-APDownload protocol
IHC protocol for KLC1 antibody 19028-1-APDownload protocol
IF protocol for KLC1 antibody 19028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FASEB J

Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.

Authors - Amélie Robert
  • KO Validated
View Article