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验证数据展示

  • WB analysis of HeLa using 18887-1-AP

    WB analysis of HeLa using 18887-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18887-1-AP (KLF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 18887-1-AP

    IF Staining of A431 using 18887-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using KLF1 antibody (18887-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

KLF1 Polyclonal antibody

KLF1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18887-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Krueppel-like factor 1, Krueppel like factor 1, Erythroid krueppel-like transcription factor, EKLF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18887-1-AP targets KLF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KLF1 fusion protein Ag13467 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Kruppel-like factor 1 (erythroid)
别名 Krueppel-like factor 1, Krueppel like factor 1, Erythroid krueppel-like transcription factor, EKLF
计算分子量 362 aa, 38 kDa
GenBank蛋白编号BC033580
基因名称 KLF1
Gene ID (NCBI) 10661
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13351
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLF1 antibody 18887-1-APDownload protocol
IF protocol for KLF1 antibody 18887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols