验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11881-1-AP targets KLF10 in WB, IP, IF, IHC, Chip, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IF, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC, Chip |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, canine |
| 免疫原 | KLF10 fusion protein Ag2482 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Kruppel-like factor 10 |
| 别名 | EGR alpha, EGRA, KLF10, Krueppel like factor 10, Kruppel like factor 10, TIEG, TIEG 1, TIEG1 |
| 计算分子量 | 469 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC011538 |
| 基因名称 | KLF10 |
| Gene ID (NCBI) | 7071 |
| RRID | AB_2877801 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13118 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLF10 (Krueppel-like factor 10), also known as TIEG1, is a transcription factor that plays a role in the regulation of cell growth. It binds to the consensus sequence 5'-GGTGTG-3'. KLF10 plays a role in the response to TGFbeta (through the TGFbeta/Smad signalling pathway) and estrogen. In both cases, KLF10 induces or repressed gene transcription to enhance the TGFbeta/Smad signalling pathway, as well as other pathways, to regulate cell proliferation, differentiation and apoptosis. KLF10 has been shown to have a role in skeletal disease (osteopenia/osteoporosis), hypertrophic cardiomyopathy, and breast and prostate cancer.This antibody is a rabbit polyclonal antibody raised against a full-length human KLF10 protein, and specifically recognizes the 52kd KLF10 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF10 antibody 11881-1-AP | Download protocol |
| IF protocol for KLF10 antibody 11881-1-AP | Download protocol |
| IP protocol for KLF10 antibody 11881-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Polymorphism rs7278468 is associated with Age-related cataract through decreasing transcriptional activity of the CRYAA promoter. | ||
J Mol Cell Cardiol Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation. | ||
Acta Histochem KLF10 is upregulated in osteoarthritis and inhibits chondrocyte proliferation and migration by upregulating Acvr1 and suppressing inhbb expression. | ||
Cell Death Discov Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis | ||
Cell Signal Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis |
