验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, rat liver tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18352-1-AP targets KLF13 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, rat, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | KLF13 fusion protein Ag13200 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Kruppel-like factor 13 |
| 别名 | BTE binding protein 3, BTEB3, FKLF2, KLF13, Krueppel like factor 13, Kruppel like factor 13, NSLP1, RFLAT 1, RFLAT1, Transcription factor BTEB3, Transcription factor NSLP1 |
| 计算分子量 | 31 kDa |
| 观测分子量 | 37-45 kDa |
| GenBank蛋白编号 | BC012741 |
| 基因名称 | KLF13 |
| Gene ID (NCBI) | 51621 |
| RRID | AB_2132399 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2Y9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kruppel-like factor 13(KLF13) is a member of transcription actors, which contains 3 zinc finger DNA-binding domains. Theses transcription factors bind to CG-rich sequences and related GT and CACCC boxes. KLF13 involved in activation of transcription of variety of genes in the cells of erythroid lineage, such as GATA1, glycophorin B. It also binds to the A and A/B RANTES promoter binding sites, the same as NFKB, and then transactivates the RANTES gene. The calculated molecular weight of KLF13 is 31 kDa, but the modified KLF13 protein is about 37-45 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF13 antibody 18352-1-AP | Download protocol |
| IHC protocol for KLF13 antibody 18352-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hepatocell Carcinoma Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma. | ||
J Biol Chem Krüppel-Like Factor 13 is a major mediator of Glucocorticoid Receptor-Signaling in Cardiomyocytes and protects these Cells from DNA Damage and Death. | ||
Placenta TET3-mediated novel regulatory mechanism affecting trophoblast invasion and migration: Implications for preeclampsia development | ||
Hum Cell Downregulation of miR-96 suppresses the profibrogenic functions of cardiac fibroblasts induced by angiotensin II and attenuates atrial fibrosis by upregulating KLF13.
| ||
Cell Biosci KLF13 suppresses the proliferation and growth of colorectal cancer cells through transcriptionally inhibiting HMGCS1-mediated cholesterol biosynthesis.
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