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KLF15 Polyclonal antibody
KLF15 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells, 3T3-L1 cells, C2C12 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| ChIP | See 1 publications below |
产品信息
13749-1-AP targets KLF15 in WB, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, ChIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | KLF15 fusion protein Ag4690 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Kruppel-like factor 15 |
| Synonyms | KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15 |
| Calculated Molecular Weight | 416 aa, 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC036733 |
| Gene Symbol | KLF15 |
| Gene ID (NCBI) | 28999 |
| RRID | AB_2132428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UIH9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptonal activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.Although the calculated molecular mass of KLF15 is 44 kDa, the apparent molecular mass on SDS-PAGE is 65 kDa due to the post-translational modifications (PMID:10982849,PMID:16615998).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF15 antibody 13749-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. | ||
Mol Cell Biol A remodeled Hsp90 molecular chaperone ensemble with the novel co-chaperone Aarsd1 is required for muscle differentiation. | ||