验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, bEnd.3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23384-1-AP targets KLF2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | KLF2 fusion protein Ag20019 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Kruppel-like factor 2 (lung) |
| 别名 | KLF2, Krueppel like factor 2, Kruppel like factor 2 (lung), LKLF, Lung krueppel like factor |
| 计算分子量 | 355 aa, 37 kDa |
| 观测分子量 | 37-40 kDa, 60 kDa |
| GenBank蛋白编号 | BC071983 |
| 基因名称 | KLF2 |
| Gene ID (NCBI) | 10365 |
| RRID | AB_3085710 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q9Y5W3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLF2 (Kruppel-like Factor 2) belongs to zinc finger transcription factors, and it is implicated in a variety of biochemical processes, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis (PMID: 17904406). KLF2 was identified as a negative regulator of T cell activation and adipogenesis. KLF2 also acts as a novel regulator of endothelial proinflammatory activation, inhibiting the inflammatory cytokine-mediated induction of adhesion molecule expression and immune cell adhesion (PMID:15718498). KLF2-null mice exhibit abnormal blood vessel formation, resulting in embryonic haemorrhage and death (PMID: 32132543). KLF2 can be detected 60 kDa by post-translationally modified (PMID: 36466816).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF2 antibody 23384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cardiothorac Surg Mechanism of KLF2 in young mice with pneumonia induced by Streptococcus pneumoniae | ||
Drug Dev Res KLF2 Inhibits Ferroptosis and Improves Mitochondrial Dysfunction in Chondrocyte Through SIRT1/GPX4 Signaling to Improve Osteoarthritis | ||
J Cell Mol Med Single-cell and spatial transcriptomics reveal apelin/APJ pathway's role in microvessel formation and tumour progression in hepatocellular carcinoma |