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KLF4 Polyclonal antibody

KLF4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11880-1-AP

Print datasheet

Synonyms

EZF, GKLF, KLF4, Krueppel like factor 4, Kruppel like factor 4 (gut)



经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HT-1080 cells, NCCIT cell
Positive IP detected inHeLa cells
Positive IHC detected inmouse embryo tissue, human tonsil tissue, human spleen tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman embronic stem cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

产品信息

11880-1-AP targets KLF4 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications chIP, FC, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF4 fusion protein Ag2480
Full Name Kruppel-like factor 4 (gut)
Synonyms EZF, GKLF, KLF4, Krueppel like factor 4, Kruppel like factor 4 (gut)
Calculated molecular weight 504 aa, 54 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC030811
Gene symbol KLF4
UniprotO43474
Gene ID (NCBI) 9314
RRIDAB_10640807
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4[PMID:15561714], with MW range from 50 to 60kDa,and major band is 55kDa. KLF4 protein can be ubiquitin modified.

实验方案

Product Specific Protocols
WB protocol for KLF4 antibody 11880-1-APDownload protocol
IHC protocol for KLF4 antibody 11880-1-APDownload protocol
IP protocol for KLF4 antibody 11880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Biol Sci

SRC-3 protects intestine from DSS-induced colitis by inhibiting inflammation and promoting goblet cell differentiation through enhancement of KLF4 expression.

Authors - Wenbo Chen
humanWB

Toxicol Appl Pharmacol

Multi-walled carbon nanotubes promoted lipid accumulation in human aortic smooth muscle cells.

Authors - Haiyin Yang
mouseWB

J Cell Biochem

SP1 regulates KLF4 via SP1 binding motif governed by DNA methylation during odontoblastic differentiation of human dental pulp cells.

Authors - Zheyi Sun
mouseWB,IF,IP

J Bone Miner Res

Klf4 Promotes Dentinogenesis and Odontoblastic Differentiation via Modulation of TGF-β Signaling Pathway and Interaction With Histone Acetylation.

Authors - Huangheng Tao
humanWB,chIP

Biochim Biophys Acta

The histone methyltransferase SETD1A regulates thrombomodulin transcription in vascular endothelial cells.

Authors - Zilong Li
  • KD Validated
humanWB

Biochem Biophys Res Commun

Krüppel-like factor 4 synergizes with CREB to increase the activity of apolipoprotein E gene promoter in macrophages.

Authors - Simona Stavri