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- KD/KO Validated
KLF4 Polyclonal antibody
KLF4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, IHC, IP, WB,ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, HT-1080 cells, NCCIT cell |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse embryo tissue, human tonsil tissue, human spleen tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human embronic stem cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
产品信息
11880-1-AP targets KLF4 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF, IHC, IP, WB,ELISA |
| Cited Applications | chIP, FC, IF, IHC, IP, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLF4 fusion protein Ag2480 |
| Full Name | Kruppel-like factor 4 (gut) |
| Synonyms | EZF, GKLF, KLF4, Krueppel like factor 4, Kruppel like factor 4 (gut) |
| Calculated molecular weight | 504 aa, 54 kDa |
| Observed molecular weight | 50-60 kDa |
| GenBank accession number | BC030811 |
| Gene symbol | KLF4 |
| Uniprot | O43474 | Gene ID (NCBI) | 9314 |
| RRID | AB_10640807 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4[PMID:15561714], with MW range from 50 to 60kDa,and major band is 55kDa. KLF4 protein can be ubiquitin modified.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF4 antibody 11880-1-AP | Download protocol |
| IHC protocol for KLF4 antibody 11880-1-AP | Download protocol |
| IP protocol for KLF4 antibody 11880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci SRC-3 protects intestine from DSS-induced colitis by inhibiting inflammation and promoting goblet cell differentiation through enhancement of KLF4 expression. | ||
Toxicol Appl Pharmacol Multi-walled carbon nanotubes promoted lipid accumulation in human aortic smooth muscle cells. | ||
J Cell Biochem SP1 regulates KLF4 via SP1 binding motif governed by DNA methylation during odontoblastic differentiation of human dental pulp cells. | ||
J Bone Miner Res Klf4 Promotes Dentinogenesis and Odontoblastic Differentiation via Modulation of TGF-β Signaling Pathway and Interaction With Histone Acetylation. | ||
Biochim Biophys Acta The histone methyltransferase SETD1A regulates thrombomodulin transcription in vascular endothelial cells.
| ||
Biochem Biophys Res Commun Krüppel-like factor 4 synergizes with CREB to increase the activity of apolipoprotein E gene promoter in macrophages. |
