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验证数据展示

  • WB analysis using 66850-1-Ig (same clone as 66850-1-PBS)

    WB analysis using 66850-1-Ig (same clone as 66850-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66850-1-Ig (KLF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    WB analysis of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    WB result of KLF5 antibody (66850-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLF5 transfected HeLa cells. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    FC experiment of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human KLF5 (66850-1-Ig, Clone:2C11C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KLF5 Monoclonal antibody, PBS Only

KLF5 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 66850-1-PBS

产品说明书

CloneNo. 2C11C5

宿主/亚型

Mouse / IgG1

种属反应性

mouse, human

应用

Indirect ELISA, WB

别名

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66850-1-PBS targets KLF5 in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 mouse, human
免疫原 KLF5 fusion protein Ag15403 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Kruppel-like factor 5 (intestinal)
别名 Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2
计算分子量 457 aa, 51 kDa
观测分子量 51-57 kDa
GenBank蛋白编号BC042131
基因名称 KLF5
Gene ID (NCBI) 688
RRIDAB_2882190
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13887
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.