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  • Featured Product
  • KD/KO Validated

KLF5 Monoclonal antibody, PBS Only

KLF5 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2C11C5

Cat no : 66850-1-PBS

Print datasheet

Synonyms

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

验证数据展示

  • WB analysis using 66850-1-Ig (same clone as 66850-1-PBS)

    WB analysis using 66850-1-Ig (same clone as 66850-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66850-1-Ig (KLF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    WB analysis of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    WB result of KLF5 antibody (66850-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLF5 transfected HeLa cells. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    FC experiment of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human KLF5 (66850-1-Ig, Clone:2C11C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

产品信息

66850-1-PBS targets KLF5 in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity mouse, human
Immunogen KLF5 fusion protein Ag15403 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Kruppel-like factor 5 (intestinal)
Synonyms Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51-57 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13887
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.