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验证数据展示

  • Sandwich ELISA standard curve of MP00155-2

    Sandwich ELISA standard curve of MP00155-2

    Sandwich ELISA standard curve of MP00155-2, Human KLF5 Recombinant Matched Antibody Pair - PBS only. 83205-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15267. 83205-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Sample test of MP00155-2

    Sample test of MP00155-2

    Hela and A549 cell lysates were measured. The human KLF5 concentration of detected samples was determined to be 7,055.7 pg/mL (based on a 3.2 mg/mL extract load) in Hela cell lyaste and 6,173.2 pg/mL (based on a 2.2 mg/mL extract load) in A549 cell lysate.

  • Cytometric bead array standard curve of MP00155-2

    Cytometric bead array standard curve of MP00155-2

    Cytometric bead array standard curve of MP00155-2, KLF5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83205-1-PBS. Detection antibody: 83205-2-PBS. Standard: Ag15267. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83205-2-PBS

    Affinity and Kinetic Characterization of 83205-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83205-2-PBS against Human KLF5 was performed. The affinity constant is below 1 pM.

KLF5 Recombinant antibody, PBS Only (Detector)

KLF5 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83205-2-PBS

Print datasheet

CloneNo. 230509C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83205-2-PBS targets KLF5 as part of a matched antibody pair:

MP00155-2: 83205-1-PBS capture and 83205-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen KLF5 fusion protein Ag15267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Kruppel-like factor 5 (intestinal)
Synonyms Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2
Calculated Molecular Weight 457 aa, 51 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13887
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C