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验证数据展示

  • Sandwich ELISA standard curve of MP00155-2

    Sandwich ELISA standard curve of MP00155-2

    Sandwich ELISA standard curve of MP00155-2, Human KLF5 Recombinant Matched Antibody Pair - PBS only. 83205-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15267. 83205-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Sample test of MP00155-2

    Sample test of MP00155-2

    Hela and A549 cell lysates were measured. The human KLF5 concentration of detected samples was determined to be 7,055.7 pg/mL (based on a 3.2 mg/mL extract load) in Hela cell lyaste and 6,173.2 pg/mL (based on a 2.2 mg/mL extract load) in A549 cell lysate.

  • Cytometric bead array standard curve of MP00155-2

    Cytometric bead array standard curve of MP00155-2

    Cytometric bead array standard curve of MP00155-2, KLF5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83205-1-PBS. Detection antibody: 83205-2-PBS. Standard: Ag15267. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83205-2-PBS

    Affinity and Kinetic Characterization of 83205-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83205-2-PBS against Human KLF5 was performed. The affinity constant is below 1 pM.

KLF5 Recombinant antibody, PBS Only (Detector)

KLF5 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83205-2-PBS

产品说明书

CloneNo. 230509C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83205-2-PBS targets KLF5 as part of a matched antibody pair:

MP00155-2: 83205-1-PBS capture and 83205-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 KLF5 fusion protein Ag15267 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Kruppel-like factor 5 (intestinal)
别名 Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2
计算分子量 457 aa, 51 kDa
GenBank蛋白编号BC042131
基因名称 KLF5
Gene ID (NCBI) 688
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13887
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C