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Mouse IgG2a isotype control Monoclonal antibody

Mouse IgG2a isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Megathura crenulata

Applications

FC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

11A1B2

Cat No : 66360-2-Ig

Print datasheet

Synonyms

Mouse IgG2a, Mouse IgG2a isotype control

验证数据展示

  • WB analysis using 66360-2-Ig

    WB analysis using 66360-2-Ig

    Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 66360-2-Ig

    FC experiment of HepG2 using 66360-2-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Mouse IgG2a isotype control (66360-2-Ig, Clone: 11A1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Cytochrome c Mouse McAb (66264-1-Ig, Clone: 2D8D11) (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected in
Positive FC detected in

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66360-2-Ig targets Mouse IgG2a isotype control in WB, IP, FC, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsFC, IP
Tested Reactivity Megathura crenulata
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Mouse IgG2a isotype control
Synonyms Mouse IgG2a, Mouse IgG2a isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

实验方案

Product Specific Protocols
WB protocol for Mouse IgG2a isotype control antibody 66360-2-IgDownload protocol
FC protocol for Mouse IgG2a isotype control antibody 66360-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
FC

Biosens Bioelectron

Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes.

Authors - Lei Wang
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IP

Nature

CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1

Authors - Milan Hluchý
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