验证数据展示
经过测试的应用
Positive IHC detected in | mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
13905-1-AP targets KLHDC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | KLHDC3 fusion protein Ag4879 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | kelch domain containing 3 |
Synonyms | PEAS, Kelch domain-containing protein 3, hPEAS |
Calculated Molecular Weight | 382 aa, 43 kDa |
GenBank Accession Number | BC041793 |
Gene Symbol | KLHDC3 |
Gene ID (NCBI) | 116138 |
RRID | AB_2877985 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BQ90 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. The Kelch domain-containing protein 3 (KLHDC3), also designated PEAS, contains 5 Kelch repeats and may be involved in meiotic recombination process. The gene encoding KLHDC3 maps to chromosome 6, which contains around 1,200 genes within 170 million base pairs of sequence. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer suggesting the presence of a cancer susceptibility locus. Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome, 21-hydroxylase deficiency and maple syrup urine disease are also associated with genes on chromosome 6. KLHDC3 protein is mainly expressed in cytoplasm, also found in meiotic chromatin.
实验方案
Product Specific Protocols | |
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IHC protocol for KLHDC3 antibody 13905-1-AP | Download protocol |
IF protocol for KLHDC3 antibody 13905-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation | ||
bioRxiv The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation |