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验证数据展示

  • WB analysis of COLO 320 using 17117-1-AP

    WB analysis of COLO 320 using 17117-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17117-1-AP (KLHL11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

KLHL11 Polyclonal antibody

KLHL11 Polyclonal Antibody for WB, ELISA
Cat No. 17117-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

kelch like 11 (Drosophila), Kelch like protein 11, KLHL11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17117-1-AP targets KLHL11 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 KLHL11 fusion protein Ag10805 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 11 (Drosophila)
别名 kelch like 11 (Drosophila), Kelch like protein 11, KLHL11
计算分子量 708 aa, 80 kDa
观测分子量 80 kDa
GenBank蛋白编号BC034470
基因名称 KLHL11
Gene ID (NCBI) 55175
RRIDAB_2130733
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVR0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHL11 is a component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating ubiquitination of target proteins. The presence of antibodies directed against KLHL11 has been identified as biomarker of a paraneoplastic neurological syndrome. The downregulation of KLHL11 may contribute to the disease progression, owing to its putative function in protein quality control. The calculated molecular weight of KLHL11 is 80 kDa (PMID: 34312900).

实验方案

Product Specific Protocols
WB protocol for KLHL11 antibody 17117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols