• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

KLHL14 Polyclonal antibody

KLHL14 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16693-1-AP

Print datasheet

Synonyms

Protein interactor of Torsin-1A, Printor, Protein interactor of torsinA, KIAA1384, Kelch-like protein 14

验证数据展示

  • WB analysis of PC-3 using 16693-1-AP

    WB analysis of PC-3 using 16693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16693-1-AP

    IF Staining of HepG2 using 16693-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16693-1-AP (KLHL14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16693-1-AP targets KLHL14 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KLHL14 fusion protein Ag10065 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 14 (Drosophila)
Synonyms Protein interactor of Torsin-1A, Printor, Protein interactor of torsinA, KIAA1384, Kelch-like protein 14
Calculated Molecular Weight 44 kDa, 71 kDa
Observed Molecular Weight35 kDa, 70 kDa
GenBank Accession NumberBC021267
Gene Symbol KLHL14
Gene ID (NCBI) 57565
RRIDAB_2131169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2G3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kelch-like protein 14 (KLHL14) is a member of the KLHL family. The kelch motif was initially discovered in Kelch. In this protein there are six copies of the motif. It has been shown for one member that it is related to Galactose Oxidase for which a structure has been solved. Two isoforms of this protein exist - 70kDa and 43kDa.

实验方案

Product Specific Protocols
WB protocol for KLHL14 antibody 16693-1-APDownload protocol
IF protocol for KLHL14 antibody 16693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols