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验证数据展示

  • WB analysis using 29253-1-AP

    WB analysis using 29253-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29253-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 29253-1-AP

    IF Staining of HepG2 using 29253-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KLHL14 antibody (29253-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

KLHL14 Polyclonal antibody

KLHL14 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29253-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Protein interactor of Torsin-1A, Printor, Protein interactor of torsinA, KIAA1384, Kelch-like protein 14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29253-1-AP targets KLHL14 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 KLHL14 fusion protein Ag29188 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 14 (Drosophila)
别名 Protein interactor of Torsin-1A, Printor, Protein interactor of torsinA, KIAA1384, Kelch-like protein 14
计算分子量 44 kDa, 71 kDa
观测分子量43 kDa, 70 kDa
GenBank蛋白编号BC021267
基因名称 KLHL14
Gene ID (NCBI) 57565
RRIDAB_3669680
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2G3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kelch-like protein 14 (KLHL14) is a member of the KLHL family. The kelch motif was initially discovered in Kelch. In this protein there are six copies of the motif. It has been shown for one member that it is related to Galactose Oxidase for which a structure has been solved. Two isoforms of this protein exist - 70kDa and 43kDa.

实验方案

Product Specific Protocols
WB protocol for KLHL14 antibody 29253-1-APDownload protocol
IF protocol for KLHL14 antibody 29253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols