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KLHL14 Polyclonal antibody

KLHL14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51131-1-AP

Print datasheet

Synonyms

kelch like 14 (Drosophila), Kelch like protein 14, KIAA1384, KLHL14

验证数据展示

  • WB analysis of HEK-293 using 51131-1-AP

    WB analysis of HEK-293 using 51131-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51131-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 51131-1-AP

    IHC staining of human ovary tumor using 51131-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51131-1-AP (KLHL14 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51131-1-AP targets KLHL14 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 14 (Drosophila)
Synonyms kelch like 14 (Drosophila), Kelch like protein 14, KIAA1384, KLHL14
Calculated Molecular Weight 44 kDa, 71 kDa
Observed Molecular Weight 55 kDa, 70 kDa
GenBank Accession NumberBC021267
Gene Symbol KLHL14
Gene ID (NCBI) 57565
RRIDAB_2131168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2G3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL14 antibody 51131-1-APDownload protocol
IHC protocol for KLHL14 antibody 51131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols