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KLHL15 Polyclonal antibody

KLHL15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25066-1-AP

Print datasheet

Synonyms

kelch like 15 (Drosophila), Kelch like protein 15, KIAA1677, KLHL15

验证数据展示

  • IHC staining of human prostate cancer using 25066-1-AP

    IHC staining of human prostate cancer using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25066-1-AP (KLHL15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 25066-1-AP

    WB analysis of HeLa using 25066-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25066-1-AP (KLHL15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25066-1-AP

    IHC staining of human testis using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25066-1-AP targets KLHL15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen KLHL15 fusion protein Ag16878 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 15 (Drosophila)
Synonyms kelch like 15 (Drosophila), Kelch like protein 15, KIAA1677, KLHL15
Calculated Molecular Weight 604 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC109058
Gene Symbol KLHL15
Gene ID (NCBI) 80311
RRIDAB_2879880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL15 antibody 25066-1-APDownload protocol
IHC protocol for KLHL15 antibody 25066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Micropeptide PACMP inhibition elicits synthetic lethal effects by decreasing CtIP and poly(ADP-ribosyl)ation.

Authors - Chuanchao Zhang
View Article
humanWB

Sci Adv

VGLL3 modulates chemosensitivity through promoting DNA double-strand break repair

Authors - Wei Wu
  • KD Validated
View Article