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验证数据展示

  • IHC staining of human prostate cancer using 25066-1-AP

    IHC staining of human prostate cancer using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25066-1-AP (KLHL15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 25066-1-AP

    WB analysis of HeLa using 25066-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25066-1-AP (KLHL15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25066-1-AP

    IHC staining of human testis using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens).

KLHL15 Polyclonal antibody

KLHL15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25066-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

kelch like 15 (Drosophila), Kelch like protein 15, KIAA1677, KLHL15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25066-1-AP targets KLHL15 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 KLHL15 fusion protein Ag16878 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 15 (Drosophila)
别名 kelch like 15 (Drosophila), Kelch like protein 15, KIAA1677, KLHL15
计算分子量 604 aa, 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC109058
基因名称 KLHL15
Gene ID (NCBI) 80311
RRIDAB_2879880
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96M94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL15 antibody 25066-1-APDownload protocol
IHC protocol for KLHL15 antibody 25066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Micropeptide PACMP inhibition elicits synthetic lethal effects by decreasing CtIP and poly(ADP-ribosyl)ation.

Authors - Chuanchao Zhang
View Article
humanWB

Sci Adv

VGLL3 modulates chemosensitivity through promoting DNA double-strand break repair

Authors - Wei Wu
  • KD Validated
View Article