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KLHL20 Polyclonal antibody

KLHL20 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18111-1-AP

Print datasheet

Synonyms

kelch like 20 (Drosophila), Kelch like protein 20, Kelch like protein X, KHLHX, KLEIP, KLHL20, KLHLX, RP3 383J4.3

验证数据展示

  • WB analysis of HEK-293 using 18111-1-AP

    WB analysis of HEK-293 using 18111-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18111-1-AP

    IHC staining of human kidney using 18111-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 18111-1-AP

    IHC staining of human kidney using 18111-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 18111-1-AP is KLHL20 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KLHL20 fusion protein Ag12567 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 20 (Drosophila)
Synonyms kelch like 20 (Drosophila), Kelch like protein 20, Kelch like protein X, KHLHX, KLEIP, KLHL20, KLHLX, RP3 383J4.3
Calculated Molecular Weight 609 aa, 68 kDa
Observed Molecular Weight 65-67 kDa
GenBank Accession NumberBC063418
Gene Symbol KLHL20
Gene ID (NCBI) 27252
RRIDAB_2131189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2M5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL20 antibody 18111-1-APDownload protocol
IHC protocol for KLHL20 antibody 18111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols