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验证数据展示

  • WB analysis using 27482-1-AP

    WB analysis using 27482-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27482-1-AP (KLHL25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27482-1-AP

    IHC staining of mouse brain using 27482-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27482-1-AP (KLHL25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 27482-1-AP

    IF Staining of A549 using 27482-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using KLHL25 antibody (27482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

KLHL25 Polyclonal antibody

KLHL25 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27482-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ENC 2, ENC2, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27482-1-AP targets KLHL25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 KLHL25 fusion protein Ag25765 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 25 (Drosophila)
别名 ENC 2, ENC2, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25
观测分子量66 kDa
GenBank蛋白编号BC028100
基因名称 KLHL25
Gene ID (NCBI) 64410
RRIDAB_2880883
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0H3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL25 antibody 27482-1-APDownload protocol
IHC protocol for KLHL25 antibody 27482-1-APDownload protocol
IF protocol for KLHL25 antibody 27482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols