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验证数据展示

  • WB analysis using 67774-1-Ig

    WB analysis using 67774-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67774-1-Ig (KLHL25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 67774-1-Ig

    IHC staining of mouse testis using 67774-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67774-1-Ig (KLHL25 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KLHL25 Monoclonal antibody

KLHL25 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67774-1-Ig

产品说明书

CloneNo. 1D8B8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

ENC 2, ENC2, FLJ12587, FLJ45015, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HT-29 cells, HepG2 cells, NCCIT cells, MOLT-4 cells, Raji cells, Ramos cells, Daudi cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67774-1-Ig targets KLHL25 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 KLHL25 fusion protein Ag27037 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kelch-like 25 (Drosophila)
别名 ENC 2, ENC2, FLJ12587, FLJ45015, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25
观测分子量66 kDa
GenBank蛋白编号BC028100
基因名称 KLHL25
Gene ID (NCBI) 64410
RRIDAB_2918539
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H0H3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL25 antibody 67774-1-IgDownload protocol
IHC protocol for KLHL25 antibody 67774-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols