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KLHL28 Polyclonal antibody

KLHL28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25224-1-AP

Print datasheet

Synonyms

BTBD5, kelch like 28 (Drosophila), Kelch like protein 28, KLHL28

验证数据展示

  • IHC staining of human liver using 25224-1-AP

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 25224-1-AP

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 25224-1-AP

    WB analysis of Jurkat using 25224-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25224-1-AP (KLHL28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 25224-1-AP

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25224-1-AP targets KLHL28 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen KLHL28 fusion protein Ag18665 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 28 (Drosophila)
Synonyms BTBD5, kelch like 28 (Drosophila), Kelch like protein 28, KLHL28
Calculated Molecular Weight 571 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC121009
Gene Symbol KLHL28
Gene ID (NCBI) 54813
RRIDAB_2879972
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHL28 also named as BTBD5 or Kelch like protein 28 is a 571 amino acid protein, which contains one BTB domain and six kelch repeats. KLHL28 localizes in the cytoplasm and belongs to BTB/kelch-associated proteins family. KLHL28 may play a role in organizing the actin cytoskeleton of the brain cells. KLHL28 is expressed as two isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for KLHL28 antibody 25224-1-APDownload protocol
IHC protocol for KLHL28 antibody 25224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols