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  • Featured Product
  • KD/KO Validated

KLHL31 Polyclonal antibody

KLHL31 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24781-1-AP

Print datasheet

Synonyms

BKLHD6, KBTBD1, kelch like 31 (Drosophila), Kelch like protein 31, Kelch like protein KLHL, KLHL, KLHL31

验证数据展示

  • WB analysis of mouse embryo using 24781-1-AP

    WB analysis of mouse embryo using 24781-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 24781-1-AP (KLHL31 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24781-1-AP

    WB analysis using 24781-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24781-1-AP (KLHL31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24781-1-AP

    IF Staining of HepG2 using 24781-1-AP

    Immunofluorescent analysis of HepG2 cells using 24781-1-AP (KLHL31 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • WB analysis of Jurkat using 24781-1-AP

    WB analysis of Jurkat using 24781-1-AP

    WB result of KLHL31 antibody (24781-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLHL31 transfected Jurkat cells.

  • IHC staining of mouse skeletal muscle using 24781-1-AP

    IHC staining of mouse skeletal muscle using 24781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24781-1-AP (KLHL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 24781-1-AP

    IHC staining of mouse skeletal muscle using 24781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24781-1-AP (KLHL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse embryo tissue, Jurkat cells
Positive IHC detected inmouse skeletal muscle tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24781-1-AP targets KLHL31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen KLHL31 fusion protein Ag20462 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 31 (Drosophila)
Synonyms BKLHD6, KBTBD1, kelch like 31 (Drosophila), Kelch like protein 31, Kelch like protein KLHL, KLHL, KLHL31
Calculated Molecular Weight 634 aa, 70 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC137267
Gene Symbol KLHL31
Gene ID (NCBI) 401265
RRIDAB_2879718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H511
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHL31 also named as BKLHD6, KBTBD1,or Kelch like protein 31 is a 634 amino acid protein, which contains one 1 BACK domain, one BTB domain and six Kelch repeats. KLHL31 as a transcription repressor in MAPK/JNK signaling pathway to regulate cellular functions. KLHL31 is strongly expressed in skeletal muscle and weakly in heart.

实验方案

Product Specific Protocols
WB protocol for KLHL31 antibody 24781-1-APDownload protocol
IHC protocol for KLHL31 antibody 24781-1-APDownload protocol
IF protocol for KLHL31 antibody 24781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols