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验证数据展示

  • WB analysis of MCF-7 using 12698-1-AP

    WB analysis of MCF-7 using 12698-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12698-1-AP

    IHC staining of human prostate cancer using 12698-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IF Staining of PC-3 using 12698-1-AP

    IF Staining of PC-3 using 12698-1-AP

    Immunofluorescent analysis of PC-3 cells using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Kallikrein 11 Polyclonal antibody

Kallikrein 11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12698-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KLK11, KLK 11, Kallikrein-11 inactive chain 2, Kallikrein-11 inactive chain 1, Kallikrein-11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, HeLa cells, PC-3 cells, Y79 cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy KLK11 Ready-To-Use IHC Kit. KLK11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12698-1-AP targets Kallikrein 11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Kallikrein 11 fusion protein Ag3427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kallikrein-related peptidase 11
Synonyms KLK11, KLK 11, Kallikrein-11 inactive chain 2, Kallikrein-11 inactive chain 1, Kallikrein-11
Calculated Molecular Weight 250 aa, 27 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC022068
Gene Symbol KLK11
Gene ID (NCBI) 11012
RRIDAB_2877876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBX7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

实验方案

Product Specific Protocols
WB protocol for Kallikrein 11 antibody 12698-1-APDownload protocol
IHC protocol for Kallikrein 11 antibody 12698-1-APDownload protocol
IF protocol for Kallikrein 11 antibody 12698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols