验证数据展示
经过测试的应用
Positive WB detected in | MCF7 cells, HeLa cells, PC-3 cells, Y79 cells |
Positive IHC detected in | human prostate cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12698-1-AP targets Kallikrein 11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Kallikrein 11 fusion protein Ag3427 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | kallikrein-related peptidase 11 |
别名 | KLK11, KLK 11, Kallikrein-11 inactive chain 2, Kallikrein-11 inactive chain 1, Kallikrein-11 |
计算分子量 | 250 aa, 27 kDa |
观测分子量 | 30-40 kDa |
GenBank蛋白编号 | BC022068 |
基因名称 | KLK11 |
Gene ID (NCBI) | 11012 |
RRID | AB_2877876 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UBX7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for Kallikrein 11 antibody 12698-1-AP | Download protocol |
IHC protocol for Kallikrein 11 antibody 12698-1-AP | Download protocol |
IF protocol for Kallikrein 11 antibody 12698-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |