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KLK15 Polyclonal antibody

KLK15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27515-1-AP

Print datasheet

Synonyms

ACO, ACO protease, HSRNASPH, Kallikrein 15, KLK15

验证数据展示

  • IHC staining of human liver cancer using 27515-1-AP

    IHC staining of human liver cancer using 27515-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 27515-1-AP

    IHC staining of human liver cancer using 27515-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of SMMC-7721 using 27515-1-AP

    WB analysis of SMMC-7721 using 27515-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27515-1-AP (KLK15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSMMC-7721 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27515-1-AP targets KLK15 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen KLK15 fusion protein Ag26232 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kallikrein-related peptidase 15
Synonyms ACO, ACO protease, HSRNASPH, Kallikrein 15, KLK15
Calculated Molecular Weight 255 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC069480
Gene Symbol KLK15
Gene ID (NCBI) 55554
RRIDAB_2880895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2R5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLK15 antibody 27515-1-APDownload protocol
IHC protocol for KLK15 antibody 27515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols