KLK3/PSA Monoclonal antibody

KLK3/PSA Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60338-1-Ig

产品说明书

CloneNo. 4A1E2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSA Ready-To-Use IHC Kit. PSA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 6 publications below

产品信息

60338-1-Ig targets KLK3/PSA in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 KLK3/PSA fusion protein Ag1060 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kallikrein-related peptidase 3
别名 KLK3, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein, EC:3.4.21.77
计算分子量 29 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC005307
基因名称 KLK3
Gene ID (NCBI) 354
RRIDAB_2881447
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07288
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for KLK3/PSA antibody 60338-1-IgDownload protocol
IHC protocol for KLK3/PSA antibody 60338-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells

Authors - Rong Wang
humanWB

J Cell Commun Signal

Estrogens drive the endoplasmic reticulum-associated degradation and promote proto-oncogene c-Myc expression in prostate cancer cells by androgen receptor/estrogen receptor signaling

Authors - Yalcin Erzurumlu
humanWB

Cell Signal

1,25(OH)2 D3 induced vitamin D receptor signaling negatively regulates endoplasmic reticulum-associated degradation (ERAD) and androgen receptor signaling in human prostate cancer cells

Authors - Yalcin Erzurumlu
humanWB

J Nat Med

Potent carotenoid astaxanthin expands the anti-cancer activity of cisplatin in human prostate cancer cells

Authors - Yalcin Erzurumlu
humanWB

Cell Signal

Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells

Authors - Yalcin Erzurumlu
humanWB

ACS Appl Mater Interfaces

Two-in-One Polymeric NO-Donor Prodrugs Mediate Precision and Synergistic Prostate Cancer Treatment

Authors - Bingbing Zhao
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