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KLK4 Polyclonal antibody

KLK4 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26547-1-AP

Print datasheet

Synonyms

ARM1, EMSP, EMSP1, Kallikrein 4, Kallikrein like protein 1, kallikrein related peptidase 4, KLK L1, KLK4, PROSTASE, PRSS17, PSTS, Serine protease 17

验证数据展示

  • IP experiment of DU 145 using 26547-1-AP

    IP experiment of DU 145 using 26547-1-AP

    IP result of anti-KLK4 (IP:26547-1-AP, 4ug; Detection:26547-1-AP 1:300) with DU 145 cells lysate 2200ug.

  • IHC staining of human prostate hyperplasia using 26547-1-AP

    IHC staining of human prostate hyperplasia using 26547-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26547-1-AP (KLK4 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human prostate hyperplasia using 26547-1-AP

    IHC staining of human prostate hyperplasia using 26547-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26547-1-AP (KLK4 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive IP detected inDU 145 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26547-1-AP is KLK4 Fusion Protein expressed in E. coli.

Tested Applications IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen KLK4 fusion protein Ag24240 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kallikrein-related peptidase 4
Synonyms ARM1, EMSP, EMSP1, Kallikrein 4, Kallikrein like protein 1, kallikrein related peptidase 4, KLK L1, KLK4, PROSTASE, PRSS17, PSTS, Serine protease 17
Calculated Molecular Weight 254 aa, 27 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC069325
Gene Symbol KLK4
Gene ID (NCBI) 9622
RRIDAB_2880548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5K2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for KLK4 antibody 26547-1-APDownload protocol
IP protocol for KLK4 antibody 26547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Am J Pathol

Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis

Authors - Jingwen Shi
View Article