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NKG2A Polyclonal antibody

NKG2A Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 10935-1-AP

Print datasheet

Synonyms

KLRC1, NKG2-A/B-activating NK receptor, NKG2, NK cell receptor A, CD159A

验证数据展示

  • WB analysis of human liver using 10935-1-AP

    WB analysis of human liver using 10935-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 10935-1-AP (NKG2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 10935-1-AP

    IHC staining of human tonsillitis using 10935-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10935-1-AP (NKG2A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 10935-1-AP

    IHC staining of human tonsillitis using 10935-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10935-1-AP (NKG2A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 10935-1-AP

    IF Staining of human tonsillitis using 10935-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using NKG2A antibody (10935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10935-1-AP targets NKG2A in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen NKG2A fusion protein Ag1376 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name killer cell lectin-like receptor subfamily C, member 1
Synonyms KLRC1, NKG2-A/B-activating NK receptor, NKG2, NK cell receptor A, CD159A
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC012550
Gene Symbol NKG2A
Gene ID (NCBI) 3821
RRIDAB_2249683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26715
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLRC1, also known as NKG2A (NKG2) and CD159A, is a single-pass type II membrane protein with extracellular C-type lectin domain. It belongs to the natural killer cell lectin-like receptor family, which is a group of transmembrane proteins preferentially expressed in NK cells. KLRC1 can form a heterodimer with CD94. The NKG2A/CD94 complex is an inhibitory receptor that recognizes HLA-E and is expressed by NK cells and a subset of T cells.

实验方案

Product Specific Protocols
WB protocol for NKG2A antibody 10935-1-APDownload protocol
IHC protocol for NKG2A antibody 10935-1-APDownload protocol
IF protocol for NKG2A antibody 10935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

PLoS One

Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia (ANKL): A Multicenter Retrospective Analysis.

Authors - Yi Li
View Article

Med

Targeting HLA-E-overexpressing cancers with a NKG2A/C switch receptor

Authors - Michelle Sætersmoen
View Article