验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10935-1-AP targets NKG2A in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | NKG2A fusion protein Ag1376 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | killer cell lectin-like receptor subfamily C, member 1 |
| 别名 | KLRC1, NKG2-A/B-activating NK receptor, NKG2, NK cell receptor A, CD159A |
| 计算分子量 | 26 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC012550 |
| 基因名称 | NKG2A |
| Gene ID (NCBI) | 3821 |
| RRID | AB_2249683 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P26715 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLRC1, also known as NKG2A (NKG2) and CD159A, is a single-pass type II membrane protein with extracellular C-type lectin domain. It belongs to the natural killer cell lectin-like receptor family, which is a group of transmembrane proteins preferentially expressed in NK cells. KLRC1 can form a heterodimer with CD94. The NKG2A/CD94 complex is an inhibitory receptor that recognizes HLA-E and is expressed by NK cells and a subset of T cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NKG2A antibody 10935-1-AP | Download protocol |
| IHC protocol for NKG2A antibody 10935-1-AP | Download protocol |
| IF protocol for NKG2A antibody 10935-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia (ANKL): A Multicenter Retrospective Analysis. | ||