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KLRF1 Polyclonal antibody

KLRF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21510-1-AP

Print datasheet

Synonyms

Activating coreceptor NKp80, CLEC5C, KLRF1, Lectin like receptor F1, ML, NKp80

验证数据展示

  • WB analysis of human spleen using 21510-1-AP

    WB analysis of human spleen using 21510-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 21510-1-AP (KLRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 21510-1-AP

    IHC staining of human tonsillitis using 21510-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21510-1-AP (KLRF1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 21510-1-AP

    IHC staining of human tonsillitis using 21510-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21510-1-AP (KLRF1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21510-1-AP is KLRF1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen KLRF1 fusion protein Ag16008 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name killer cell lectin-like receptor subfamily F, member 1
Synonyms Activating coreceptor NKp80, CLEC5C, KLRF1, Lectin like receptor F1, ML, NKp80
Calculated Molecular Weight 231 aa, 27 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC098257
Gene Symbol KLRF1
Gene ID (NCBI) 51348
RRIDAB_2878873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZS2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLRF1 antibody 21510-1-APDownload protocol
IHC protocol for KLRF1 antibody 21510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols