• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

    WB analysis of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83812-1-RR (KMO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83812-1-RR (same clone as 83812-1-PBS)

    IHC staining of mouse brain using 83812-1-RR (same clone as 83812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 83812-1-RR (same clone as 83812-1-PBS)

    IHC staining of mouse heart using 83812-1-RR (same clone as 83812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 83812-1-RR (same clone as 83812-1-PBS)

    IHC staining of mouse kidney using 83812-1-RR (same clone as 83812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 83812-1-RR (same clone as 83812-1-PBS)

    IHC staining of mouse kidney using 83812-1-RR (same clone as 83812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

    FC experiment of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KMO Recombinant antibody (83812-1-RR, Clone:240586D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83812-1-RR

    Affinity and Kinetic Characterization of 83812-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83812-1-RR against Human KMO were performed. The affinity constant is below 1 pM.

KMO Recombinant antibody, PBS Only

KMO Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83812-1-PBS

产品说明书

CloneNo. 240586D3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83812-1-PBS targets KMO in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 KMO fusion protein Ag35029 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
别名 Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9
计算分子量486 aa, 56 kDa
观测分子量52-56 kDa
GenBank蛋白编号BC005297
基因名称 KMO
Gene ID (NCBI) 8564
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15229
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).