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验证数据展示

  • WB analysis using 28695-1-AP

    WB analysis using 28695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28695-1-AP (KNL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 28695-1-AP

    IF Staining of U2OS using 28695-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KNL1 antibody (28695-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

KNL1 Polyclonal antibody

KNL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28695-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CASC5, D40, CT29, Cancer/testis antigen 29, Blinkin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28695-1-AP targets KNL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen KNL1 fusion protein Ag29881 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cancer susceptibility candidate 5
Synonyms CASC5, D40, CT29, Cancer/testis antigen 29, Blinkin
Calculated Molecular Weight 265 kDa
Observed Molecular Weight300 kDa
GenBank Accession NumberNM_170589
Gene Symbol CASC5/KNL1
Gene ID (NCBI) 57082
RRIDAB_3086079
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG31
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KNL1 is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. KNL1 functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues.

实验方案

Product Specific Protocols
WB protocol for KNL1 antibody 28695-1-APDownload protocol
IF protocol for KNL1 antibody 28695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols