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KNOP1 Polyclonal antibody

KNOP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30115-1-AP

Print datasheet

Synonyms

101F10.1, C16orf88, FAM178A, Protein C16orf88, TSG118

验证数据展示

  • WB analysis using 30115-1-AP

    WB analysis using 30115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30115-1-AP (KNOP1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 30115-1-AP

    IF Staining of MCF-7 using 30115-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KNOP1 antibody (30115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inK-562 cells, U-251 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30115-1-AP targets KNOP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen KNOP1 fusion protein Ag32395 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 16 open reading frame 88
Synonyms 101F10.1, C16orf88, FAM178A, Protein C16orf88, TSG118
Calculated Molecular Weight52 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_001012991.2
Gene Symbol KNOP1
Gene ID (NCBI) 400506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ1ED39
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lysine-rich nucleolar protein 1 (KNOP1) is a nucleolar protein that interacts with zinc finger 106 protein. Also known as Tsg118, it was first reported in 1999 (3). KNOP1 is predominantly expressed in proliferating somatic cells and in male germ cells, and may therefore be involved in testicular development. (PMID: 37588747)

实验方案

Product Specific Protocols
WB protocol for KNOP1 antibody 30115-1-APDownload protocol
IF protocol for KNOP1 antibody 30115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols