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验证数据展示

  • WB analysis using 30115-1-AP

    WB analysis using 30115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30115-1-AP (KNOP1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 30115-1-AP

    IF Staining of MCF-7 using 30115-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KNOP1 antibody (30115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

KNOP1 Polyclonal antibody

KNOP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30115-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

101F10.1, C16orf88, FAM178A, Protein C16orf88, TSG118

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, U-251 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:14000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30115-1-AP targets KNOP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 KNOP1 fusion protein Ag32395 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 16 open reading frame 88
别名 101F10.1, C16orf88, FAM178A, Protein C16orf88, TSG118
计算分子量52 kDa
观测分子量60 kDa
GenBank蛋白编号NM_001012991.2
基因名称 KNOP1
Gene ID (NCBI) 400506
RRIDAB_3086234
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ1ED39
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lysine-rich nucleolar protein 1 (KNOP1) is a nucleolar protein that interacts with zinc finger 106 protein. Also known as Tsg118, it was first reported in 1999 (3). KNOP1 is predominantly expressed in proliferating somatic cells and in male germ cells, and may therefore be involved in testicular development. (PMID: 37588747)

实验方案

Product Specific Protocols
WB protocol for KNOP1 antibody 30115-1-APDownload protocol
IF protocol for KNOP1 antibody 30115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols