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验证数据展示

  • WB analysis using 24694-1-AP

    WB analysis using 24694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24694-1-AP (KNTC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

KNTC1 Polyclonal antibody

KNTC1 Polyclonal Antibody for WB, ELISA
Cat No. 24694-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Kinetochore-associated protein 1, Kinetochore associated protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NCI-H1299 cells
Planning an IHC experiment? We recommend our IHCeasy KNTC1 Ready-To-Use IHC Kit. KNTC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24694-1-AP targets KNTC1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 KNTC1 fusion protein Ag20268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinetochore associated 1
别名 Kinetochore-associated protein 1, Kinetochore associated protein 1
计算分子量251 kDa
观测分子量210-230 kDa
GenBank蛋白编号BC150278
基因名称 KNTC1
Gene ID (NCBI) 9735
RRIDAB_3669445
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50748
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KNTC1 is a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Northern blot analysis revealed low-to-moderate expression in all tissues tested, with highest expression in testis.

实验方案

Product Specific Protocols
WB protocol for KNTC1 antibody 24694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols