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KPNA3 Polyclonal antibody

KPNA3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28050-1-AP

Print datasheet

Synonyms

SRP1 gamma, Qip2, Karyopherin subunit alpha-3, Karyopherin subunit alpha 3, Importin subunit alpha-4

验证数据展示

  • WB analysis using 28050-1-AP

    WB analysis using 28050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28050-1-AP (KPNA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28050-1-AP

    IHC staining of mouse brain using 28050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28050-1-AP (KPNA3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA3 antibody included
  • IHC staining of human brown disease using 28050-1-AP

    IHC staining of human brown disease using 28050-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28050-1-AP (KPNA3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA3 antibody included
  • IF Staining of HepG2 using 28050-1-AP

    IF Staining of HepG2 using 28050-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KPNA3 antibody (28050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inmouse brain tissue, human brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA3 Ready-To-Use IHC Kit. KPNA3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28050-1-AP targets KPNA3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen KPNA3 fusion protein Ag27755 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name karyopherin alpha 3 (importin alpha 4)
Synonyms SRP1 gamma, Qip2, Karyopherin subunit alpha-3, Karyopherin subunit alpha 3, Importin subunit alpha-4
Calculated Molecular Weight 58 kDa
Observed Molecular Weight57-60 kDa
GenBank Accession NumberBC017355
Gene Symbol KPNA3
Gene ID (NCBI) 3839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00505
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KPNA3, karyopherin subunit alpha 3, also known as SRP1 or IPOA4. It is expected to be located in cytoplasm and nucleus, the protein is ubiquitous and highly expressed in heart, skeletal muscle. The calculated molecular weight of KPNA3 is 57 kDa. Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin.

实验方案

Product Specific Protocols
WB protocol for KPNA3 antibody 28050-1-APDownload protocol
IHC protocol for KPNA3 antibody 28050-1-APDownload protocol
IF protocol for KPNA3 antibody 28050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols