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验证数据展示

  • WB analysis using 28050-1-AP

    WB analysis using 28050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28050-1-AP (KPNA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28050-1-AP

    IHC staining of mouse brain using 28050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28050-1-AP (KPNA3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA3 antibody included
  • IHC staining of human brown disease using 28050-1-AP

    IHC staining of human brown disease using 28050-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28050-1-AP (KPNA3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA3 antibody included
  • IF Staining of HepG2 using 28050-1-AP

    IF Staining of HepG2 using 28050-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KPNA3 antibody (28050-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

KPNA3 Polyclonal antibody

KPNA3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28050-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Importin alpha Q2, Importin subunit alpha-4, Karyopherin subunit alpha 3, Karyopherin subunit alpha-3, Qip2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inmouse brain tissue, human brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA3 Ready-To-Use IHC Kit. KPNA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28050-1-AP targets KPNA3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 KPNA3 fusion protein Ag27755 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 karyopherin alpha 3 (importin alpha 4)
别名 Importin alpha Q2, Importin subunit alpha-4, Karyopherin subunit alpha 3, Karyopherin subunit alpha-3, Qip2
计算分子量 58 kDa
观测分子量57-60 kDa
GenBank蛋白编号BC017355
基因名称 KPNA3
Gene ID (NCBI) 3839
RRIDAB_3086026
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00505
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KPNA3, karyopherin subunit alpha 3, also known as SRP1 or IPOA4. It is expected to be located in cytoplasm and nucleus, the protein is ubiquitous and highly expressed in heart, skeletal muscle. The calculated molecular weight of KPNA3 is 57 kDa. Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin.

实验方案

Product Specific Protocols
WB protocol for KPNA3 antibody 28050-1-APDownload protocol
IHC protocol for KPNA3 antibody 28050-1-APDownload protocol
IF protocol for KPNA3 antibody 28050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols