Cytokeratin 1-specific Polyclonal antibody
Cytokeratin 1-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | rat skin tissue, mouse skin |
Positive IP detected in | A431 cells |
Positive IHC detected in | human skin tissue, human lung cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16848-1-AP targets Cytokeratin 1-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | IF, IHC, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, goat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | keratin 1 |
Synonyms | 67 kDa cytokeratin, CK 1, CK1, Cytokeratin 1, Cytokeratin 1-specific, EHK1, Hair alpha protein, keratin 1, KRT1, KRT1A, KRTA |
Calculated Molecular Weight | 66 kDa |
Observed Molecular Weight | 67 kDa |
GenBank Accession Number | NM_006121 |
Gene Symbol | KRT1 |
Gene ID (NCBI) | 3848 |
RRID | AB_2134417 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P04264 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Cytokeratin 1-specific antibody 16848-1-AP | Download protocol |
IHC protocol for Cytokeratin 1-specific antibody 16848-1-AP | Download protocol |
IF protocol for Cytokeratin 1-specific antibody 16848-1-AP | Download protocol |
IP protocol for Cytokeratin 1-specific antibody 16848-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
J Invest Dermatol Nuclear IL-33 plays an important role in the suppression of filaggrin, loricrin, keratin 1, and keratin 10 by IL-4 and IL-13 in human keratinocytes. | ||
J Invest Dermatol IL-22 downregulates peptidylarginine deiminase-1 in human keratinocytes: adding another piece to the IL-22 puzzle in epidermal barrier formation. | ||
Int J Mol Sci Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm. | ||
Sci Rep Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis. | ||
Front Endocrinol (Lausanne) c-Myc Upregulated by High Glucose Inhibits HaCaT Differentiation by S100A6 Transcriptional Activation. |