KRT12 Polyclonal antibody
KRT12 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy KRT12 Ready-To-Use IHC Kit. KRT12 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 6 publications below |
产品信息
24789-1-AP targets KRT12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | KRT12 fusion protein Ag20312 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | keratin 12 |
| Synonyms | CK 12, Cytokeratin 12, K12, keratin 12, KRT12 |
| Calculated Molecular Weight | 494 aa, 54 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC156641 |
| Gene Symbol | KRT12 |
| Gene ID (NCBI) | 3859 |
| RRID | AB_2879726 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99456 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KRT12 antibody 24789-1-AP | Download protocol |
| IHC protocol for KRT12 antibody 24789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea. | ||
Exp Eye Res Low-dose repeated exposure to chemical surfactant impairs corneal epithelium: When personal cleaning products entering the eye. | ||
Int Ophthalmol ZD6474 attenuates TGF-β1-induced fibrosis in human Tenon fibroblasts and inhibits neovascularization via AKT-mTOR signaling pathway | ||
J Ocul Pharmacol Ther ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway | ||
Transl Vis Sci Technol Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500 |