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验证数据展示

  • WB analysis using 22221-1-AP

    WB analysis using 22221-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 22221-1-AP

    IHC staining of human skin using 22221-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with KRT14 antibody included
  • IHC staining of human skin using 22221-1-AP

    IHC staining of human skin using 22221-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22221-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with KRT14 antibody included
  • IP experiment of mouse skin using 22221-1-AP

    IP experiment of mouse skin using 22221-1-AP

    IP result of anti-Cytokeratin 14 (IP:22221-1-AP, 4ug; Detection:22221-1-AP 1:1000) with mouse skin tissue lysate 3200ug.

  • IF Staining of mouse skin using 22221-1-AP

    IF Staining of mouse skin using 22221-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 14 antibody (22221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skin using 22221-1-AP

    IF Staining of mouse skin using 22221-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 14 antibody (22221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human bowen disease using 22221-1-AP

    IHC staining of human bowen disease using 22221-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22221-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT14 antibody included

Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 22221-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF-P, IP, ELISA and More (1)

别名

KRT14, keratin 14, Cytokeratin-14, Cytokeratin,Keratin, CK-14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skin tissue
Planning an IHC experiment? We recommend our IHCeasy KRT14 Ready-To-Use IHC Kit. KRT14 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22221-1-AP targets Cytokeratin 14 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, monkey, hamster
免疫原 Cytokeratin 14 fusion protein Ag17559 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 14
别名 KRT14, keratin 14, Cytokeratin-14, Cytokeratin,Keratin, CK-14
计算分子量 472 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC002690
基因名称 Cytokeratin 14
Gene ID (NCBI) 3861
RRIDAB_2879036
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis. This antibody is specifically against KRT14.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 14 antibody 22221-1-APDownload protocol
IHC protocol for Cytokeratin 14 antibody 22221-1-APDownload protocol
IF protocol for Cytokeratin 14 antibody 22221-1-APDownload protocol
IP protocol for Cytokeratin 14 antibody 22221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, hamsterWB

Cancer Gene Ther

Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model.

Authors - S Vijayalingam
View Article
mouse,monkeyIF

Invest Ophthalmol Vis Sci

Single-Cell Transcriptomic Analysis Reveals Dynamic Cellular Processes in Corneal Epithelium During Wound Healing in Cynomolgus Monkeys

Authors - Ming Zhou
View Article